A generalized study of human sperm DNA fragmentation between Asthenozoospermia and Normozoospermia semen sample

Abstract

Objective: A generalized study of human sperm DNA fragmentation between Asthenozoospermia and Normozoospermia semen sample. Design: An observational study of human sperm DNA fragmentation in semen samples carried out during my master's dissertation.  Settings: Institute of Reproduction and Child Cares & IRCC IVF Centre, Panchkula, Haryana. Patient Selection: All male infertility patients visited for semen analysis. Those patients whose consent was not obtained weren't included in this study, and Azoospermic patients were excluded as well. Duration of Study: 3 Months. Outcome: Sperm DNA fragmentation percentage was evaluated in Asthenozoospermia and Normozoospermia and recorded differences. Results: In this observational research, 31 patients were included who had semen analysis for infertility therapy at the Institute of Reproduction and Child Cares & IVF Center between March 2021 and May 2021.20 of the individuals (64.51%) were taken into consideration for the sperm DNA fragmentation test.Rest patients 11 (35.48 %) wasn't given consent for research purpose; we excluded those patients from this study. During Sperm DNA fragmentation evaluation, we found degraded (type a) was 3.7 %, without halo (type b) 4.28 %, Medium halo (type c) 11.73% and Big halo (type d) 5.29 % in AZS males and degraded (type a) was 2.34 %, without halo (type b) 3.89 %, Medium halo (type c) 45.54 % and Big halo (type d) 23.23 % in NZS males. Conclusion: The mean of sperm with Sperm DNA fragmentation (SDF) [degraded (a) + without halo (b) = 160.60%] was found in Asthenozoospermia, and the mean of sperm with SDF [degraded (a) + without halo (b) = 124.60%] in Normozoospermia.