OBSERVATIONAL STUDY OF HUMAN SPERM SURVIVAL & MOTILITY IN TWO DIFFERENT MEDIUMS: A COMPARABLE ANALYSIS

Abstract

Objective: To compare the rate of human sperm survival and motility in two different mediums (HTF and CSC) during infertility treatment in sibling’s sample. Methodology: In this two-way observational research, the semen quality for infertility therapy was assessed in 45 patients who received infertility treatment at the Institute of Reproduction and Child Cares & IRCC IVF Center in Panchkula, Haryana. The study was conducted between December 2021 and June 2022. The semen analysis, preparation of semen, and survival of sperm were considered for 20 of these patients (44.44%). The remaining 12 patients (26.66%) had poor sperm counts and motility and were excluded from the study. Moreover, 13 (28.88 %) patients, did not agree to give consent for this study and they were excluded from this review. Semen planning was done in view of sperm motility and count . We mixed one pellet in the 2-ml HTF medium and another one in 2 ml CSC medium and observe sperm survival and motility after 12 hours of sperm preparation in sibling’s semen sample also, recorded the sperm endurance and motility. Results: We saw that sperm endurance and motility were 66.40 % in HTF medium and 84.25 % in CSC medium. According to the results of the comparison between HTF and CSC, there was a 17.85 % difference in sperm survival and motility. Summary: Based on the results of this study, the sperm's survival and motility rates are higher in CSC medium than HTF medium. In order to improve the survival and motility of sperm, more accurate and reliable methods are required.